5

Isolation of brain

experimental animals were killed by cervical dislocation and the

brain was removed. The brain was dissected on a glass plate over

ice

and

striatum

brain

region

was

removed

as

outlined

by

Glowinski

immediately

and

Iversen

[6].

The

in

brain

region

was

weighed

tissue

and

homogenized

a

Potter-Elvehjem

homogenizer.

Assay of AChE activity

AChE

activity

was

of

determined

following

the

spectrophotometric

method

(Sigma

Ellman

et

USA)

al.,

as

[3]

employing

eserine

sulfate

Chemicals,

inhibitor

and

acetylthiocholine iodide as substrate.

Protein content and statistical analysis

method

of

Lowry

et al.,

Chemicals,

[9]

with

bovine

serum

albumin

as

was

standard

expressed

(Sigma

USA).

AChE

enzyme

Analysis

activity

as

nanomoles/min/mg

protein.

of

variance

coupled with post hoc student `t' test was used for statistical

evaluation.