KUMAR6

method of Ohkawaet al., (1979). The reaction mixture contained in

a

final volume of 4.0 ml, 0.2 ml of 8.1% sodium dodecyl sulfate and 1.5 ml

of thiobarbituric acid. The mixture was heated in waterbath for 60 min,

cooled and 1.0 ml of distilled water was added. The absorbance of the

solution was measured at 532 nm.

Protein determination and statistical analysis

(1951) using bovine serum albumin as standard. AChE enzyme activity of

tissue homogenate was expressed as nanomoles/min/mg protein and LPO level

as nanomoles/mg protein. The results were statistically analyzed using

two sample `t' test and the mean values were tested at significant levels

of p<0.05-p<0.001.

RESULTS

Statistical

differences

in

AChE

activity

on

aluminium

exposure

within

each

brain

region

are

presented

in

Fig

1. AChE

activity

was

significantly decreased (p<0.05-p<0.001) in the brain regions. Decreased

AChE

activity

in

hippocampus

(16%)

and

mid

brain

(11%)

were

not

be

and

statistically significant. Decrease

in

AChE

activity

was

found

to

highest in olfactory lobe followed by medulla oblongata, striatum

cortex.