KUMAR5

volume

administered

at

dose

of

320

mg/kg

kept

body

weight.

The

final

administered to the rats were

received deionized water.

Tissue preparation

constant

at

1.0

ml.

Control

rats

Animals

were

sacrificed

by

decapitation.

The

brain

was

quickly

isolated and dissected on a glass plate over ice and the brain regions

were dissected as outlined by Glowinski and Iversen (1966). The brain

regions were weighed immediately and

homogenized

in

a

Potter-Elvehjem

tissue homogenizer (20 mg of tissue/ml). The homogenate was centrifuged

and the supernatant was used for the biochemical assay.

Determination of AChE activity

acetylthiocholine

iodide

in

0.1

M

sodium

phosphate

buffer

(pH

8.0)

according to the method of Ellman et al.,(1961). The rate of increase in

absorbance (412 nm) was recorded for 5 min in the cuvette compartment of

a Bausch and Lomb spectrophotometer. Correction was made for nonenzymatic

hydrolysis of substrate using eserine sulfate as inhibitor.

Measurement of lipid peroxidation level

Lipid peroxidation level of the brain regions was determined

by

assay of malonaldehyde in 0.25 M sucrose tissue homogenate following the